Electrochemical Method for Detecting Hippuric Acid Using Osmium-antigen Conjugate on the Gold Nanoparticles Modified Screen-printed Carbon Electrodes

Choi, Young-Bong; Kim, Hyug-Han

doi:2011.2.1.57

J. Electrochem. Sci. Technol > Volume 2(1); 2011 > Article

Article

Journal of Electrochemical Science and Technology 2011;2(1):57-61.
DOI: https://doi.org/10.5229/JECST.2011.2.1.057

Electrochemical Method for Detecting Hippuric Acid Using Osmium-antigen Conjugate on the Gold Nanoparticles Modified Screen-printed Carbon Electrodes

Young-Bong Choi, Hyug-Han Kim

Department of chemistry, Dankook University;Department of chemistry, Dankook University;

Abstract

This paper describes an electrochemical immunoassay for simple, fast and quantitative detection of a urinary hippuric acid which is one of major biological indicator in toluene-exposed humans. The electrochemical system of immunoassay was based on the directly osmium complex conjugated with hippuric acid. With the competition between free hippuric acid (HA) and the osmium-hippuric acid conjugate (Os-HA) to bind with antibody hippuric acid (Anti-HA) coated onto gold nanoparticles, the electrical signals were proportional to urinary hippuric acid (HA) in the range of 0.01-5 mg/mL which is enough range to be used for in-field or point-of-care (POC) diagnosis. The proposed electrochemical method can be extended to the applications to detect a wide range of different small molecules in the field of health care.

Keywords: Immunosensor, hippuric acid, osmium, point-of-care